Here is a summary of a great new email subject line analysis from YesWare:
- Blank subject lines are very effective (but I find this to be sneaky so avoid)
- Avoid using subject lines with questions
- Avoid exclamation marks in subject lines
- Use “Re:” (legit replies only please; avoid doing this with first emails since too sneaky)
- Include numbers (though uplift is minor)
- Avoid greeting words in subject lines (ex: Hi, Hey)
- Avoid explicit event invite in subject lines (“invitation”, “webinar”, “join us”, …)
- Avoid urgency words in subject lines (“exclusive”, “last chance”, …)
- Use Title Case (caps on first letter of each word)
Source: http://pages.yesware.com/rs/344-PYD-331/images/Email-Subject-Lines-That-Actually-Work.pdf